stratagene pCR-script--- good or bad?

jlanders at hmivax.humgen.upenn.edu jlanders at hmivax.humgen.upenn.edu
Sun Apr 10 12:52:05 EST 1994


In article <Cnuxvs.53M at ucdavis.edu>, john hess <jfhess at ucdavis.edu> writes:
> 
> Howdy folks, 
> 
> Can any users of the above mentioned cloning vector/ kit post some
> opinions concerning performance?  I am currently cloning into pSP72 using
> the pharmacia sureclone kit and have found that it works fine but gives
> colonies without inserts a fair proportion of the time.  
> 
> thanks,
> 
> John Hess, PhD                    Phone me 916 752 8420
> Dept of Human Anatomy             FAX me 916 752 8520
> University of Calif               Email me jfhess at ucdavis.edu
> Davis, CA                         or leave me alone, your choice.


I have not used pCR Script but I have used the TA vector PCR II from In
vitrogen.  This product works great!  My only suggestion if you use it though
is that you should phenol:chloroform your PCR product, run it on a gel and
glass extract the product.  Good luck!



More information about the Methods mailing list