stratagene pCR-script--- good or bad?

jlanders at jlanders at
Sun Apr 10 12:52:05 EST 1994

In article <Cnuxvs.53M at>, john hess <jfhess at> writes:
> Howdy folks, 
> Can any users of the above mentioned cloning vector/ kit post some
> opinions concerning performance?  I am currently cloning into pSP72 using
> the pharmacia sureclone kit and have found that it works fine but gives
> colonies without inserts a fair proportion of the time.  
> thanks,
> John Hess, PhD                    Phone me 916 752 8420
> Dept of Human Anatomy             FAX me 916 752 8520
> University of Calif               Email me jfhess at
> Davis, CA                         or leave me alone, your choice.

I have not used pCR Script but I have used the TA vector PCR II from In
vitrogen.  This product works great!  My only suggestion if you use it though
is that you should phenol:chloroform your PCR product, run it on a gel and
glass extract the product.  Good luck!

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