What is nitrocellulose?
James B. Hutchins
jbh at anat.UMSMED.EDU
Mon Apr 11 16:26:21 EST 1994
In article <2o4mqo$ntt at crcnis1.unl.edu> mgriep at unlinfo.unl.edu (m griep)
>What is the chemical structure of nitrocellulose and
>why do proteins stick to it?
>My second question is: why do proteins stick to it so well?
Try this reference:
Van Oss CJ. Good RJ. Chaudhury MK.
Mechanism of DNA (Southern) and protein (Western) blotting on cellulose
nitrate and other membranes.
Journal of Chromatography. 391(1):53-65, 1987 Mar 27.
I also recommend it highly to anyone doing nucleic acid or protein blotting
who wants to *understand* the procedures, rather than merely *following*
Hope it helps!
Jim Hutchins  E-Mail: jbh at anat.umsmed.edu
Asst Prof of Anatomy  Asst Prof of Neurology
Univ Mississippi Med Ctr  Jackson, MS
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