Klenow question

Steven Coon at umich.edu
Mon Apr 11 11:53:37 EST 1994


In article <sticknbd-080494070634 at 160.129.125.9>,
sticknbd at ctrvax.vanderbilt.edu (Henry) wrote:

> When I make random-primed probes, the protocol I use
> says to stop the reaction after 30 to 60 minutes with
> EDTA (or heat), which I always do.  What would be the
> consequences of allowing the reaction to continue?  I
> cannot envision any ill effects of letting the reaction
> go, even if it were to go on for several hours.  Comments?
> sticknbd at miranda.cc.vanderbilt.edu



I think that the enzyme doesn't last for very long  anyway so it really
doesn't matter very much. In most cases the majority of labeling occurs in
the first 15 minutes anyway.

Steve Coon
sdcoon at umich.edu
University of Michigan Medical Center.



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