Help purifying large amounts protein

Dan Diaz bl275 at cleveland.Freenet.Edu
Mon Apr 11 11:08:06 EST 1994


In a previous article, botjlh at lure.latrobe.edu.au () says:

>G'day Netters,
>	I am attempting to purify large amounts of protein (>1mg) of 35 KDa
>from two other proteins of 42 KDa and 78KDa. I cannot use immunoaffinity or
>affinity chromatography. Neither can I use denaturing PAG's in the Bio-Rad prep
>cell.
>
>Any ideas would be greatly appreciated.
>
>Thanks,
>Joshua
>botjlh at lure.latrobe.edu.au
>

try:
anion/cation exchange chromatography
hydroxylapatite
hydrophobic interaction
size exclusion
chromatofocusing
ammonium sulfate precipitation
phase partitioning
ultrafiltration
exhaustive size-exclusion dialysis



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