Help purifying large amounts protein

Dan Diaz bl275 at cleveland.Freenet.Edu
Mon Apr 11 11:08:06 EST 1994

In a previous article, botjlh at () says:

>G'day Netters,
>	I am attempting to purify large amounts of protein (>1mg) of 35 KDa
>from two other proteins of 42 KDa and 78KDa. I cannot use immunoaffinity or
>affinity chromatography. Neither can I use denaturing PAG's in the Bio-Rad prep
>Any ideas would be greatly appreciated.
>botjlh at

anion/cation exchange chromatography
hydrophobic interaction
size exclusion
ammonium sulfate precipitation
phase partitioning
exhaustive size-exclusion dialysis

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