How do you make Deionized foramide?
M K Bennett
pamkb at mail.bris.ac.uk
Mon Apr 11 06:58:18 EST 1994
Michael Coady (coady at ERE.UMontreal.CA) wrote:
: In article <2o3hkm$p6b at mercury.hgmp.mrc.ac.uk> mlush at crc.ac.uk (Mr. M.J. Lush) writes:
: > The subject says it all really, we want it for Southern
: >and Northen work, and are tired of having to wait for it from
: >CloneTech, (lower cost would be another plus point...)
: According to Maniatis, just sit your formamide with
: Dowex XG8 mixed-bed resin for one hour and filter twice through
: Whatman #1 paper. Store deionized formamide in small aliquots
: under nitrogen at -70 C. Another resin is Bio-Rad's AG501-X8(D )
: (20-50 mesh). Use 5 g /100 ml.
: P.S. This stuff is in every basic lab book, like Maniatis or
: Current Protocols or Met. Enzymol. 152. It's pretty easy to
: find, and you'll probably feel more confident seeing it there
: rather than in one of the postings here.
: Michael J. Coady
: COADY at ERE.UMONTREAL.CA
: The opinions expressed above are solely those of the author and do not,
: in any way, shape or form, represent the Universite de Montreal.
This probably goes against the grain somewhat but I find that most of the
formamide that you buy doesnt need to be deionised unless it has a slight
yellow colour to it. I generally dont bother and have had no problems
with my Northerns or Southerns.
University of Bristol
m.k.bennett at bristol.ac.uk
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