Help purifying large amounts protein

botjlh at lure.latrobe.edu.au botjlh at lure.latrobe.edu.au
Sun Apr 10 23:34:25 EST 1994


G'day Netters,
	I am attempting to purify large amounts of protein (>1mg) of 35 KDa
from two other proteins of 42 KDa and 78KDa. I cannot use immunoaffinity or
affinity chromatography. Neither can I use denaturing PAG's in the Bio-Rad prep
cell.

Any ideas would be greatly appreciated.

Thanks,
Joshua
botjlh at lure.latrobe.edu.au



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