dextran sulfate-cheap substitute

David Ish-Horowicz D_HOROWICZ at icrf.icnet.uk
Tue Apr 12 14:39:40 EST 1994


> In article <1994Apr12.124209.1 at hkucc.hku.hk>, h9290184 at hkucc.hku.hk writes:
> > In article <Cnt28w.IuG at news.cis.umn.edu>, mman at molbio.cbs.umn.edu (Michael Man) writes:
> >>  	I use dextran sulfate in my hybridization solution.  It gives me very 
> >> good results, but it is too expensive, $732 per 500 gms from Sigma.  Does 
> >> anyone know a cheaper place to go?  Thanks for your help.
> >> 	Also I will be very grateful if you can suggest an alternative way 
> >> to prepare prehyb. and hyb. solution.  I use GeneScreen transfer membrane and
> >> UV-crosslink RNA. 
> >> 
> >> 
> >> Michael
> >> mman at molbio.cbs.umn.edu

... and other messages

Try using polyethylene glycol 8000 (50 fold-cheaper?). You should try 6%
and 10% as I'm not sure which is better.

David Ish-Horowicz
ICRF Developmental Biology Unit



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