Enzyme Kinetics Question

Warren Gallin wgallin at gpu.srv.ualberta.ca
Tue Apr 12 16:41:25 EST 1994


In Article <9404111941.AA22231 at unixg.ubc.ca>, flo at UNIXG.UBC.CA wrote:
>Netters,
>
>If an enzyme has such an incredibly low activity on a substrate that you 
>need to use equal molar amouts of enzyme to substrate, how does this 
>affect the kinetics ?   ie can you still use the Michealis-Menten type 
>equations even though the enzyme concentration is no longer much smaller 
>than the substrate concentration?????


If you need to use an equimolar amount of enzyme to substrate, it isn't an
enzyme, it is a reactant.  The Michaelis Menten equation will not apply
period.  Are you sure it really is equimolar?
Warren Gallin,
Department of Zoology, University of Alberta
wgallin at gpu.srv.ualberta.ca



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