Hybridizations using degenerate oligo's

Wayne Beyer beyer at bdrc.bd.com
Wed Apr 13 08:52:24 EST 1994


We are interested in identifying a gene using a degenerate oligo.  We have 
N-terminal sequence of the protein of interest and want to use a reverse 
translated oligo to identify the gene.  We  would appreciate 
suggestions/experiences on designing, labeling, and hybridizing degenerate 
oligos.
Thanks in advance,
PTH
JAD 



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