NaOH in PCR buffer

Matthias Zeiner mzei at sun0.urz.uni-heidelberg.de
Wed Apr 13 10:18:00 EST 1994


On Wed, 13 Apr 1994 11:57:53 GMT, Fabrice Glibert wrote:

>
>hi,
>
>i've heard about using NaOH in PCR buffer for the
>amplification of GC rich region
>Does anybody have some info about this or something
>to amplify a GC rich region
>thanx

Include 10% DMSO in the reaction buffer. I tried this for a very GC rich 
template and it worked greatly.
Matthias



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