On Wed, 13 Apr 1994 11:57:53 GMT, Fabrice Glibert wrote: > >hi, > >i've heard about using NaOH in PCR buffer for the >amplification of GC rich region >Does anybody have some info about this or something >to amplify a GC rich region >thanx Include 10% DMSO in the reaction buffer. I tried this for a very GC rich template and it worked greatly. Matthias