Help with radioactive PCR

Dr. M. Rhodes mrhodes at crc.ac.uk
Wed Apr 13 05:46:38 EST 1994


Help

Iam carrying out radioactive PCR using 35S-dATP,
when I did the sums ror the method we were using 
I found that the concentratoons
 of  our NTP's
were
dCTP, dTTP, dGTP 200 uM each
whilst 
dATP was 10 uM 
this dores not seem right
what levels of NTP's do others use?
Thanks for any help
Michael Rhodes
mrhodes at crc.ac.uk
ps please reply by email if poss







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