PCR from genomic DNA

Jim Owens jow at helix.nih.gov
Thu Apr 14 15:06:42 EST 1994


In article <199404061949.AA00341 at wugate.wustl.edu> ,
krishnan at BORCIM.WUSTL.EDU writes:
>Could somebody tell me how to PCR-amplify a 3 kb piece of DNA from total
human
>genomic DNA or from total yeast DNA

In addition to what others have said:

If your DNA is very viscous, you should consider making it less viscous
by some vigorous vortex mixing or by digesting with a rare cutting
restriction enzyme that does not cut between your PCR primers.

Good luck,

Jim Owens



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