MOPS buffer usage - ? reuse for RNA gels

sticknbd at miranda.cc.vanderbilt.edu sticknbd at miranda.cc.vanderbilt.edu
Thu Apr 14 13:16:06 EST 1994


In article <2ojoqs$beg at gazette.bcm.tmc.edu>, kyoas at bcm.tmc.edu (Karen L.
Yoas) wrote:
 > I have limited amts of chemicals and I would like to know if there is
> a problem reusing my buffer (MOPS) for RNA gels.  I have heard of those
> who reuse TBE buffer over a couple of times.
 > Karen
I use TAE for running DNA on agarose up to three times with no ill
effects, but I change my 0.22M formaldehyde MOPS buffer for 
Northerns every time.  However, I do occasionally use yellowed
10X MOPS without problem.  This is probably a FAQ, but what 
exactly is the matter with yellowed MOPS anyway?
sticknbd at miranda.cc.vanderbilt.edu



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