Cloning low abundance PCR products

David Schuster 72170.3152 at COMPUSERVE.COM
Thu Apr 14 08:43:48 EST 1994


Dear netters,

Has anyone tried GIBCO BRL's UDG cloning method?   We routinely use it for
expression cloning of genes.  Normally we can capture a PCR product from genomic
DNA after 5-8 PCR cycles.  The low cycle number is used to minimize introduction
of mutations by Taq.  All comments welcome....and yes, I am affilated with
developing this technology.  I am interested in successes, failures, complaints,
whatever. 


Thanks.

David Schuster
Senior Scientist
Life Technologies, Inc.
PO Box 6009
Gaithersburg, MD 20884-9980

Ph. (301) 670-7781




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