b-galactosidase assay after transfection
HGF02633 at niftyserve.or.jp
Thu Apr 14 06:24:02 EST 1994
Does anybody know sesitive assay system to detect beta-galactosidase
activity in cell extracts after DNA transfection (lipofectin method).
We transfected pCH110 (beta-galactosidase expression vector) into PC12
cells. However, the transfection efficiency was very low. We could not
detect the enzyme activity with o-nitrophenol-b-D-galactopyranoside
(ONPG) as a substrate, though the CAT activity was detected in the same
expeirment. How can we overcome this problem. please teach me.
Department of Biochemistry, Chiba University, School of Medicine
1-8-1, Inohana, Chuo-ku, Chiba, 260 JAPAN
QFH03407 at niftyserve.or.jp
tishizuk at twics.com
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