b-galactosidase assay after transfection

石塚俊治 HGF02633 at niftyserve.or.jp
Thu Apr 14 06:24:02 EST 1994


Does anybody know sesitive assay system to detect beta-galactosidase 
activity in cell extracts after DNA transfection (lipofectin method). 
We transfected pCH110 (beta-galactosidase expression vector) into PC12 
cells. However, the transfection efficiency was very low. We could not 
detect the enzyme activity with o-nitrophenol-b-D-galactopyranoside 
(ONPG) as a substrate, though the CAT activity was detected in the same 
expeirment. How can we overcome this problem. please teach me.

Sincerely yours,

=========================================================================
 Dr.Toshiharu Ishizuka
 Department of Biochemistry, Chiba University, School of Medicine
 1-8-1, Inohana, Chuo-ku, Chiba, 260 JAPAN
 FAX +81-43-226-2041
 E-mail
 QFH03407 at niftyserve.or.jp
 tishizuk at twics.com
 =========================================================================



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