PCR probes

Martin Leach leach at mbcrr.harvard.edu
Thu Apr 14 10:24:36 EST 1994


In article <1994Apr13.125331.5635 at leeds.ac.uk>, futers at bpxtal.leeds.ac.uk
wrote:

> Hi netters,
> 
> Has anyone performed a PCR reaction in the presence of a 32P dNTP and
> then used the product to probe a Southern blot?
> If there is any interest I will post a summary.
> 
>                        Many thanks,
>                          Simon    (futers at biovax.leeds.ac.uk)

I believe many people do this - although i prefer the random priming method
with hexamers (only takes 30mins).

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
               USA                           head.....WIBBLE" -BLACKADDER 
p.s. try BioMOO (virtual biology on the internet - telnet
bioinfo.weizmann.ac.il 8888)
 



More information about the Methods mailing list