What was hydroxy-lysine?
Brian T. Greuel
GREUELB1 at JAGUAR.UOFS.EDU
Thu Apr 14 15:53:42 EST 1994
In article <2ojups$o2l at mercury.hgmp.mrc.ac.uk>, mlush at crc.ac.uk (Mr. M.J.
> We have managed to produce a limited amount of protein
> sequence by edman degredation, unfortunatly one of these contains two
> We wish to use these protein sequences to devise degenerate
> oligos so we want to know:-
> What was hydroxy-lysine (OH-lys) before it was altered?
It was lysine! Lysine and proline residues are hydroxylated in some proteins
by a specific hydroxylase enzyme which occurs in the endoplasmic reticulum.
These modifications are made co-translationally, I believe, to produce
hydroxylysine and hydroxyproline residues, respectively.
> Is this an artifact?
Of what? The Edman degradation? I don't think so.
> Has OH-lys been found in proteins _other_ than collagen?
> And if so what was it doing there?
It is also found in elastin, another extracellular matrix protein. I think
these hydroxylysine and hydroxyproline residues act to stabilize the
interchain linkages of fibrous proteins such as collagen and elastin.
Hope this helps.
More information about the Methods