Qiaex and ligations

Helge Weissig helgew at LJCRF.EDU
Thu Apr 14 16:42:03 EST 1994


Dear Guy,

I'd asume off the top of my head, that you might have some silica carried over during the elution step. I usually spin the eluate again for 5 min. and transfer it to a fresh tube.

hope this helps,
Helge


At 01:20 PM 04/14/94 +0100, LOGAN wrote:
>Dear Netters, I am posting this for a friend, but as he doesn't have a mailbox
>could you please e-mail replies to this address. logan at montpellier.inra.fr
>
>We have just switched from geneclean to Qiaex for economic reasons, but there
>seems now to be a problem with our ligations, has anyone had any similar
>experience, and any solutions/ideas? One idea is that we may not be doing the
>drying step properly, they say too long and your DNA is stuck for good, but that
>you have to get rid of the ethanol!, so maybe its residual ethanol that is the
>problem. Sorry if this has been discussed before.
>
>Thank you very much for any advice.
>
>Guy



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Helge WEISSIG                              e-mail: helgew at ljcrf.edu
La Jolla Cancer Research Foundation        phone: (619) 455 6480 x253
10901 N. Torrey Pines Rd.                  FAX: (619) 455 0181
La Jolla, CA 92037
U.S.A.

     God invented surfing, so that the really talented people
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