What was hydroxy-lysine?
David I Resnick
dresnick at athena.mit.edu
Fri Apr 15 06:30:22 EST 1994
In article <2ojups$o2l at mercury.hgmp.mrc.ac.uk> mlush at crc.ac.uk (Mr. M.J. Lush) writes:
> We have managed to produce a limited amount of protein
> sequence by edman degredation, unfortunatly one of these contains
> two hydroxy-lysines.
> We wish to use these protein sequences to devise degenerate
> oligos so we want to know:-
> What was hydroxy-lysine (OH-lys) before it was altered?
It was lysine. LysOH is occasionally glycosylated in collagen, but I
guess it wasn't in your case I guess as that would have probably made
it unidentifiable on your HPLC.
> Is this an artifact?
I dunno. I wouldn't thing so.
> Has OH-lys been found in proteins _other_ than collagen?
> And if so what was it doing there?
Hydroxylysine has also been seen in the proteins containing short (<30
gly-x-y repeats) coll. domains. E.g. secreted proteins like C1q, mannose binding
protein, conglutinin and transmembrane proteins like the macrophage
scavenger receptor. If your Edman degradations are not putting the
hydroxylysine in the Y positions of the Gly-X-Y repeat it could be
interesting, since I've not heard of it being elsewhere.
David Resnick dresnick at athena.mit.edu
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