GHOST-BAND UN-MASKED

Duncan Clark Duncan at genesys.demon.co.uk
Fri Apr 15 03:36:52 EST 1994


In article <9404141753.AA28809 at pines2.ljcrf.edu> helgew at LJCRF.EDU writes:

>The problem I have is a predominant mini-prep (handmade, no kits, no silica)
> product from Bluescript transformations. It is really the only band I get
> sometimes and in its amount comparable to 'normal' plasmid.
>Its size is somewhere around 1.5 to 1.8 kb. It is cutable with a few enzymes
> (at least appears to be cut on EtBr gels) but usually contains no insert.

Have you correlated the RE's which cut with those that are known to cleave
ss-DNA? I think NEB have a list in their catalog.

Duncan
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Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
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