library screening with degenerated oligomers
schen at fred
Fri Apr 15 12:46:37 EST 1994
I am trying to use degenrated oligomers derived from consensus amino acid sequences to screen cDNA library, and need suggestion in the following parameters.
1)how much oligomers to use in relation to the degree of degeneracy of oligomres
2)what temperature to use in the hybridization
3)the washing condition
Other tips or a working protocol are appreciated.
Seattle, Wa 98122
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