PCR probes

Shiao Y. Wang sywang at whale.st.usm.edu
Fri Apr 15 22:21:23 EST 1994


: PS Has anybody tried a capillary PCR machine? It's ace! We've got one
: on trial and we just ran a 35 cycles reaction (30 sec ext) in 23 minutes!

What is the volume of the total reaction? How easy were the reactions to
set up (does it take long to load the capillary tubes)? What about getting
the samples ready for gel electrophoresis? Are the tubes sealed and how do
you get the products out? 35 cycles in 23 minutes is quite amazing.

Shiao Wang
University of Southern Mississippi




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