Sequencing gel

[Yolanda Yang]

In article <Stephen_Lasky-130494151444 at poncho-slip13.cis.brown.edu>,
Stephen_Lasky at brown.edu (Stephen R. Lasky) wrote:

> I have had that problem when I used plates the first time after
> siliconizing them with dichlorodimethylsilane.  The gel used to crawl out
> into the top tank.  You can avoid this by washing the plates with a
> slightly abrasive powder detergent after you siliconize them.  That always
> worked for me.
> 

In our lab, we found that Rain-X (found in auto shops) is a great
substitute for silicon, and less toxic.  We avoid the "crawling" phenomenon
by only Rain-Xing the short plate, so that the gel sticks to the long
plate.

yolanda yang
yangy at rockvax.rockefeller.edu