Sequencing gels

Michael Joiner joiner at pobox.upenn.edu
Fri Apr 15 15:54:09 EST 1994


Can anyone tell me how to minimize leakage between adjacent wells when
using polyacrilamide gels with sharkstooth combs? We seem to have tried
every combination of prewarming with/without comb, new vs old combs, and
insertion depth. Whether or not we get leakage still seems to be a random
occurrence! Does everybody have this trouble?

Mike



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