UV radiation of PCR mixes

NEWLIN_C newlin_c at am.pclsv4.sandoz.com
Fri Apr 15 15:25:09 EST 1994


Add all the reagents to your PCR tube except: enzyme and DNA template.
Irradiate this on a UV lightbox for 20 minutes.  Primers are o.k. to 
add to the mixture if they are under 25 bases in length.  They will
not cross-link at this size because they are too small.  Good
luck!

C. Newlin




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