PCR

[Andreas Vogel]

hi there,
somebody recently asked:

 drabek at risc (Mgr.Jiri Drabek), Faculty Hospital, Olomouc, Czech Republic
Keywords: PCR, wax
Date: Wed, 13 Apr 1994 14:31:00

Hello, netters!
Please, does anybody know alternative (cheap, home made) for PERKIN-ELMER
AmpliWax PCR gem (used for hot start)?
Any answer will be appreciated.
 
(sorry, I don't know how to put those nice ">" next to a citation)

Yes, there's an alternative: plain paraffin wax in beads or "pastilles",
melting point 57-60C, for example from BDH, Poole, England, the kind that you
use for embedding. They look and work just like those Ampliwax things for $2
apiece, but cost only, say 
a piece, but cost only 
(oops, damn that e-mail editor!!) ... only $40 per kilo!
I've been using them for a while, and I never had any contamination problems.
 
Good luck,
Andreas

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Andreas Vogel
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