Inhibitor of ProteinaseK?

Greg Gloor ggloor at julian.uwo.ca
Fri Apr 15 11:24:56 EST 1994


Subject: Inhibitor of ProteinaseK?
From: BSS032
Date: 15 Apr 1994 16:56:32 +0100
In article <2omdfg$eaa at mserv1.dl.ac.uk> , BSS032 at BANGOR.AC.UK writes:
>Dear Netters,
>I have been doing partial proteolysis with pk, now I want to stop
>the reaction 

Proteinase K is a serine protease. So you can stop it by adding
Phenylmethylsulfonylfloride (PMSF) @ a final concentraion of 1 mM to the
reaction.
That will kill it.



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