DNA extraction from eggs

APARKER at MAINE.MAINE.EDU APARKER at MAINE.MAINE.EDU
Fri Apr 15 11:46:17 EST 1994


Howdy.

I need to extract PCR-able DNA from fish eggs that were frozen shortly
after fertilization (eg not too many cells present).  They are large,
yolky things, meaning that I need a protocol that will isolate a small
amount of DNA from a relatively large amount of lipid.  Any advice
would be greatly appreciated.

Thanks in advance,

Alex Parker

U Maine Zoology



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