PCR specificity

P Watson md1pw at sunc.sheffield.ac.uk
Sat Apr 16 08:52:21 EST 1994


Hi Amanda,

Perhaps you should check out your RT step. Maybe your working on contaminating

traces of genomic DNA. Sounds wacky but it does happen, and some controls, like

beta-actin, can lead you astray because of the presence of numerous spliced

pseudogenes. Just a thought.

Phil
.




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