b-galactosidase assay after transfection

Michael Kertesz mkertesz at micro.biol.ethz.ch
Mon Apr 18 04:03:59 EST 1994


>> Does anybody know sesitive assay system to detect beta-galactosidase 
>> activity in cell extracts after DNA transfection (lipofectin method). 
>
>I believe there may be a fluorescent substrate for bgal. Detection of this
>can be made at very low levels - i think (please correct me)
>

The reagent you are after is methylumbelliferyl galactoside (MUG). After the beta-
galactosidase reaction, the product detected is methylumbelliferone, which can be 
easily measured in a fluorimeter. We find about ten-fold increased sensitivity over
the ONPG method.

Michael Kertesz




More information about the Methods mailing list