b-galactosidase assay after transfection
mkertesz at micro.biol.ethz.ch
Mon Apr 18 04:03:59 EST 1994
>> Does anybody know sesitive assay system to detect beta-galactosidase
>> activity in cell extracts after DNA transfection (lipofectin method).
>I believe there may be a fluorescent substrate for bgal. Detection of this
>can be made at very low levels - i think (please correct me)
The reagent you are after is methylumbelliferyl galactoside (MUG). After the beta-
galactosidase reaction, the product detected is methylumbelliferone, which can be
easily measured in a fluorimeter. We find about ten-fold increased sensitivity over
the ONPG method.
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