Antigenicity of His Tag?

Steven Joseph Szarka sjszarka at acs.ucalgary.ca
Tue Apr 19 15:29:47 EST 1994


Has anyone generated antibodies against a histidine tagged
protein without cleaving the tagged peptide sequences away from
the desired sequence?

Is the his tag highly antigenic?

Would this cause problems with background when the antibody
population is used in Westerns and IPs on eukaryotes?

I would be interested in hearing any experiences from those who
have gone through this before.

Steve
sjszarka at acs.ucalgary.ca






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