ligations in LMP agarose

the End jgraham at bronze.ucs.indiana.edu
Tue Apr 19 11:09:37 EST 1994


You should remove most of the LM agarose by freezing the slice and then 
spining out the debris. This gives only a small (four-fold) reduction in
ligation-transformation efficiency as compared to samples without any
agarose (as reported to me by Mike Coady <coady at ere.umontreal.ca>). Maniatis
describes a 100-fold reduction associated with ligation in melted agarose.

See the FAQ or search the archive at ftp.bio.indiana.edu by gopher on 
"consistent and subcloning" for more details.

Sorry for the broken record effect.


Jim
J. Graham
Indiana University ............heading for St. Louis  !



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