ligations in LMP agarose

otello at otello at
Tue Apr 19 11:52:20 EST 1994

This is to reply to the post about problems  for in gel_ligations :
I think your problem is that you're using TBE as a running buffer:
Maniatis says that inhibits ligase :use TAE instead, I've done that 
even for three fragments ligations and necer had a problem



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