NaOH in PCR buffer

Wasun Chantratita asmsi002 at CMU.CHIANGMAI.AC.TH
Tue Apr 19 10:46:37 EST 1994



On 13 Apr 1994, Matthias Zeiner wrote:

> On Wed, 13 Apr 1994 11:57:53 GMT, Fabrice Glibert wrote:
> 
> >
> >hi,
> >
> >i've heard about using NaOH in PCR buffer for the
> >amplification of GC rich region
> >Does anybody have some info about this or something
> >to amplify a GC rich region
> >thanx
> 
> Include 10% DMSO in the reaction buffer. I tried this for a very GC rich 
> template and it worked greatly.
> Matthias
> 
  I never use NaOH, you may try 5-15% glycerol. We used that stuff 
successfully with various type of GC rich templates.

 Wasun> 




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