NaOH in PCR buffer
asmsi002 at CMU.CHIANGMAI.AC.TH
Tue Apr 19 10:46:37 EST 1994
On 13 Apr 1994, Matthias Zeiner wrote:
> On Wed, 13 Apr 1994 11:57:53 GMT, Fabrice Glibert wrote:
> >i've heard about using NaOH in PCR buffer for the
> >amplification of GC rich region
> >Does anybody have some info about this or something
> >to amplify a GC rich region
> Include 10% DMSO in the reaction buffer. I tried this for a very GC rich
> template and it worked greatly.
I never use NaOH, you may try 5-15% glycerol. We used that stuff
successfully with various type of GC rich templates.
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