advice needed for check trace mRNA

GEORGE XIAOXI OUYANG, ELECTRICAL ENGINEERING, PHONE 579-0970 ldeng at kean.ucs.mun.ca
Mon Apr 18 13:45:48 EST 1994


Hi! Dear Netters! I want to identify very little trace of mRNA in the cells 
by RT-PCR or Northern hybridization. I certainly want to show the RT-PCR 
signal is not come from DNA. So anyone can tell me what is the best way to 
extract RNA which is free of DNA? What is the best probe (for Lymphocytes) 
for the very-low-expression mRNA in Northern hybridization? Thanks in 
advance!  Langtuo Deng at: ldeng at kean.ucs.mun.ca



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