ligations in LMP agarose

futers at bpxtal.leeds.ac.uk futers at bpxtal.leeds.ac.uk
Wed Apr 20 04:20:38 EST 1994


In article <Martin.19.2DB3A3AD at gene.unp.ac.za>, Martin at gene.unp.ac.za
 (Darren Martin) writes:
>I am ligating 125 ng pUC18 (SmaI digested and partially end filled) with +-
                             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>50 ng insert DNA (+- 2 Kb Sau3AI digestion fragments that are also partially
[stuff deleted]

How do you partially end fill a SmaI fragment?
Just wondering.
      Simon Futers (futers at biovax.leeds.ac.uk)

>Any help will be much appreciated.
>



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