sequencing GC-rich fragment

Dmitry A. Los losda at NIBB.AC.JP
Wed Apr 20 03:37:17 EST 1994


Dear netters,
we are struggling with sequencing a 2.0 kbp DNA fragment
from gram-positive bacterium. The fragment is extremely GC-rich,
it's almost impossible to read the sequence in some regions.
We are using Applyed Biosystems automatic sequences and tried
both dye primer and dye terminators. No effect.
Any suggestions?
Thank you.

   
Dmitry A. Los
National Institute for Basic Biology
Myodaiji, Okazaki 444, Japan
fax: 81-564-54-4866
E-mail: losda at nibb.ac.jp




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