Taq and dITP

mfb102 at CD4680FS.RRZE.UNI-ERLANGEN.DE mfb102 at CD4680FS.RRZE.UNI-ERLANGEN.DE
Wed Apr 20 08:33:23 EST 1994


 Dear netters,
 just a simple question. I want to introduce random mutations in a short
 DNA sequence by PCR using  dITP instead of dGTP. Does Taq (or Vent) accept
 dITP (e.g. T7 does) ??
 Any suggestions
 Thank you
 Robert

* Robert Slany Ph.D., Institut fuer Biochemie, Fahrstr. 17, Germany *
*    Tel. 0049(GER)-9131-854196, Fax: 0049(GER)-9131-854605         *
*       mfb102 at cd4680fs.rrze.uni-erlangen.de (Internet)             *
*              ----------Don`t panic-----------                     *



More information about the Methods mailing list