Carbohydrates & Coumarin Detection
sstriepk at crl.com
Wed Apr 20 16:34:30 EST 1994
Recently I saw a New England Biolabs ad in a journal that showed a
picture of a TLC plate with coumarin 120 labelled oligosaccharides on it. I
was courious if anyone knows the method and can explain how the coumarin
labelled oligos are detected. The photo in the ad shows blue bands on a
white background which makes me think that they were not photographing
If anyone has used the method can you say what the sensitivity is?
I assume that the derivatization with coumarin 120 of the oligosaccharide
reductive amination just like F.A.C.E. and some of the pyridylamination
schemes used by others.
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