TBE buffer precipitation

Martin Leach leach at mbcrr.harvard.edu
Wed Apr 20 11:44:10 EST 1994


In article <01HBE8BNHEKI9BZS8P at delphi.com>, RMARTINQFD at BIOTECHNET.COM
wrote:

> Help!  My lab has been making 10XTBE buffer solution
> 
> 
> 
> s for years and 
> we frequently get precipitation forming.  Does anyone know the cause of this 
> problem?  How can you make up 10XTBE stock solutions that do not precipitate?
> What problems can TBE precipitation cause if we use the buffer anyway in
> our experiments (sequencing gels, agarose gels)?
> 
> Thanks in advance,
> 
> Rick in Indiana


Sometimes it does ppt. sometimes it doesnt (rare though). I use it when it
has ppt'd slightly and it works fine. A good tip to clean the ppt from the
bottle is to use glacial acetic acid ;)

Martin

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
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