Crystal Violet for Plaque Assay

Robert Citek rwcitek at
Wed Apr 20 15:11:10 EST 1994

I am doing plaque assays for polio virus.  After I remove the agar
from my 6-well TC plates, I would like to stain them with a 
solution of crystal violet.  Does any one have a recipe for CV 
for use in a plaque assay?  I have tried several microbiology
manuals and each one seems to use a slightly different mixture. 
The tissue culture manuals that I have do not even mention CV.

Thanks in advance for any and all help.

Robert Citek
University of California, Irvine
School of Social Ecology
rcitek at

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