39-mer primers

[Jenny Witherspoon]

I am current trying to use a primer that is 39 base pairs long primer in
a pcr reaction.  I have previously use 10-mers a gained good results (ie
good banding patterns ).  However when I use the 39-mers I don't get any
banding patterns.  Does any on know what PCR program and primer concentration
would best suit these primers.  Also if anyone knows of any papers that
use long primers I would appreciate the reference.  By the way I'm using
Almond DNA.
Please send any information to jwitherspoon@