TBE buffer precipitation
myirwin at uci.edu
Thu Apr 21 16:30:16 EST 1994
In article <9404210227.AA23473 at cscgpo.anu.edu.au>,
Klaus.Matthaei at ANU.EDU.AU wrote:
> >Help! My lab has been making 10XTBE buffer solution
> >s for years and
> >we frequently get precipitation forming. Does anyone know the cause of this
> >problem? How can you make up 10XTBE stock solutions that do not precipitate?
> >What problems can TBE precipitation cause if we use the buffer anyway in
> >our experiments (sequencing gels, agarose gels)?
> The answer my friends seems to be blowing in the wind : DUST??
> 0.2 micron filter the 10xTBE and it stops precipitating (at least in my > > > hands). Micronucleation of salts is my conclusion and yes if the ppte forms, then the sequencing gels don't get hot enough.
Klaus, your suggestion certainly addresses the root of the problem.
However, you can go one step further and address particulates in your
If you do a quick acid rinse of the bottle to filter into, a 20x TBE
solution will stay in solution for over a couple of months if handled
carefully. Even a 25x solution will work for a while. I suppose if you
could waste a large, plastic, all-in-one filtration unit, the results may
be even better.
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