Crystals during X-gal staining
leach at mbcrr.harvard.edu
Thu Apr 21 11:16:52 EST 1994
In article <CoMBtv.I1M at freenet.carleton.ca>, ar229 at FreeNet.Carleton.CA
(Allison Haggarty) wrote:
> I have been using X-gal to stain cells blue after transient tranfections.
> The cells are blue allright but there are lots of crystals in the stain so
> the results are not very photogenic. The problem seems to arise when the
> X-gal stock (10% in DMF, frozen at -20 C) is diluted 1:100 in the working
> buffer for staining (5mM K ferricynanide, 5mM K ferrocyanide, 2 mM MgCl2,
> made up in PBS). As soon as the X-gal hits the aqueous buffer the
> solution goes cloudy.
> Does anyone know how to prevent this? Am I stuck with filtering?
Warm it to 37C for 10-20mins then filter it
Otherwise u get crystals forming when u place it on the cultures.
..... Martin Leach Email:leach at mbcrr.harvard.edu
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