checking for DNA on nylon membranes?

Lynda Callicotte lcallico at utkvx.utk.edu
Thu Apr 21 15:22:35 EST 1994


In article <rcaspi.14.2DADD381 at ucsd.edu> rcaspi at ucsd.edu (Ron Caspi) writes:
>From: rcaspi at ucsd.edu (Ron Caspi)
>Subject: checking for DNA on nylon membranes?
>Date: Thu, 14 Apr 1994 23:36:34 GMT
>Keywords: SS DNA, membranes

>Hi everybody
>I have a question here:
>I have some nylon membranes (HiBond N) that once had DNA on them (transferred 
>by southern blotting). These membranes have been hybridized and stripped, so 
>the DNA is now single stranded. However, my last probing yielded nothing - no 
>signal whatsoever. I suspect that I might have lost the DNA.
>Does anybody know how I could check if the DNA is still there?

>My thanks for everyone who would like to help

>Ron


>*************************************************************************
>Ron Caspi      Scripps Institution of Oceanography     UC San Diego
>La Jolla  CA  92093-0202   Phone (619)534-0638 Fax (619)534-7313
>*************************************************************************

The only thing I can think of is to re-probe with something you absolutely 
know should be positive if your DNA is still there.  A vector sequence, for 
instance.

Lynda




More information about the Methods mailing list