HI HO SILVER

dkim at unm.edu dkim at unm.edu
Thu Apr 21 14:22:03 EST 1994


Re: re-amplification of silver-stained DNA:

I use the general idea described by Bassam Caetano-Annoles in BioTechniques
(Recently, but I don't have a cittion).  They just cut out the stained band
and put it directly into the PCR reaction. That's about all. It worked for
me (I got several bands of approximately the same size. These were cloned
into pGEM-T).

Daniel Kim



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