Crystals during X-gal staining
ar229 at FreeNet.Carleton.CA
Thu Apr 21 11:44:19 EST 1994
I have been using X-gal to stain cells blue after transient tranfections.
The cells are blue allright but there are lots of crystals in the stain so
the results are not very photogenic. The problem seems to arise when the
X-gal stock (10% in DMF, frozen at -20 C) is diluted 1:100 in the working
buffer for staining (5mM K ferricynanide, 5mM K ferrocyanide, 2 mM MgCl2,
made up in PBS). As soon as the X-gal hits the aqueous buffer the
solution goes cloudy.
Does anyone know how to prevent this? Am I stuck with filtering?
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