Differential Display :

David M. Berman eyeball at netcom.com
Thu Apr 21 08:43:59 EST 1994

Another inexplicably important variable is the amount of time you
allow your 1st strand synth. to sit around b/4 amplifying.  The less
time, the better.  We don't know why but have seen it several times in
different peoples hands.  One other impt parameter is input RNA
amount.  You have to do a titration with each type of RNA used.

                                             eyeball at netcom.com

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