a couple things
sticknbd at ctrvax.vanderbilt.edu
Thu Apr 21 11:17:33 EST 1994
1 Does anybody know the molecular mechansim of
DEPC inactivation of RNases?
2 I had some trouble with RNA gels I was running
recently. It seemed there was a variable amount
of precipitation in the wells, and very little 18 &
28S. Swapping out several solutions (formaldehyde
with some precipitation, yellowed 10X mops) made
no difference. What solved it was changing my Etbr.
The stuff I had been using was stored bench top at
room T. the stuff I just started using was from the
same batch (I made it up in Octover 1993) but stored
refrigerated. The bench top stuff was somewhat
more opaque and perhaps of a darker hue than the
refrigerated aliquots. Anyone care to guess what
went on with the EtBr and why/how it ruined my
gels? I never heard of EtBr going bad.
sticknbd at miranda.cc.vanderbilt.edu
More information about the Methods