Possible ghost plasmids available for study

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Fri Apr 22 12:15:07 EST 1994


| From pnh Tue Apr 19 11:20:27 1994
| To: STEHELIN at ARTHUR.CITI2.FR
| Subject: Re:  Paul N. Hengen, Ghost
 
> Dear Paul. After reading the article "Ghost plasmid of pBluescript" in the 1994
> March issue of TiBS, I went back to a gel where I had seen some "fancy" bands I
> dind' worry to much about. I think IT is the Ghost: In the 18 different digests
> of the same plasmid prep (a 6kbp insert in pBluescript SK+ grown in TG1), I see
> 2 extra faint bands (1.5 kbp and 2.9kbp) and and even fainter bands (6, 8, and 2
> which migrate very little). In he two only lanes where I don't see theese bands
> (even smaller ones), I used ScaI (alone or with SphI), the others ER (PvuII,
> SphI, HinDIII, PstI, XbaI, XhoI, EcoRI, NcoI, BamHI, BglII, HinCII, and SmaI)
> had no effect on the electrophoresis pattern of theese two bands.
> I don't have recollection of any other experience of this kind. By the way, it
> was (9-22-1993) a CsCl, Alkaline Lysis prep.
> It would be nice of you if you could let me know when you identify this Ghost,
> thanks.
>
> Patrick MARTIN, stehelin at arthur.citi2.fr
> Institut Pasteur Lille, FRANCE
> Tel: 011-33-20877878 Fax: 011-33-20877908
 
| Dear Patrick:
|  
| Thank you for the interest in my column. There are a few people on the net who
| may be following this. If you are not a current reader of the methods group,
| would you mind if I post this for others? I am not currently looking into this
| phenomenon, but someone else may be interested in your results or plasmids.
|  
| If the ghost plasmid is identified, I will definitely follow up in a future
| issue of TIBS. Thanks.
| 
| -Paul.

: SURE, you can post it , and good luck.
: Patrick MARTIN, stehelin at arthur.citi2.fr
: Institut Pasteur Lille, FRANCE
: Tel: 011-33-20877878 Fax: 011-33-20877908.
: 4/20/1994

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* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
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